Reporter

Part:BBa_K1891006:Design

Designed by: Zhao Jingyu   Group: iGEM16_BNU-China   (2016-10-10)


C-Luciferase+alpha-tubulin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 673
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We connected this two gene fragments using fusion PCR. First we acquired alpha-tubulin gene fragment and C-Luciferase gene fragment. And then we redesigned a antisense primer of C-Luciferase and a sense primer of alpha-tubulin with a linker about 42bp. And then doing fusion PCR.


Source

C-Luciferase comes from pCambia1300-C-Luciferase plasmid. Alpha-tubulin comes from human breast cancer cell line Mcf-7 cDNA.

References